FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTAG64910.32455No Hit
GCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAG54800.27399999999999997No Hit
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG52030.26015No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA46770.23384999999999997No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC43120.21559999999999999No Hit
TGGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTA33700.1685No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGC32930.16465No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA32000.16No Hit
CCCCGCTGGAGCCCTGCTGGTTGCTGAGGTAGACGCTGAAGACGGGGCTG31870.15935000000000002No Hit
GCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCTGA25900.1295No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA25580.12789999999999999No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT25290.12645No Hit
CTCACGGATAGACTTAAATTTCTTCAGGGGCACTTTGACCACTGCTGCCT24430.12215No Hit
GGGGGCTTCAATTGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA23160.1158No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA22980.1149No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC21120.10560000000000001No Hit
GCCCTAGGAGGGTGCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAG20540.10269999999999999No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA20070.10035000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)