FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000927113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA30280.1514No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAAACATCGATCTCGTAT28380.1419Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC23210.11605000000000001No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT22400.11199999999999999TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)