FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44960.2248No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44100.2205No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC38290.19145No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG36420.18209999999999998No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT30640.1532TruSeq Adapter, Index 1 (97% over 36bp)
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG29680.1484No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA28210.14105No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA25240.1262No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC24780.12390000000000001No Hit
GTAGGCTTCTTTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAA20780.10389999999999999No Hit
CTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATA20150.10074999999999999No Hit
CCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGAT20140.10070000000000001No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA20080.10039999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)