FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC46490.23245000000000002No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA43370.21685No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC37270.18635No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG36530.18265No Hit
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG29300.1465No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA27880.1394No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT25430.12715TruSeq Adapter, Index 1 (97% over 36bp)
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA24700.1235No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC24220.1211No Hit
CTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATA20760.1038No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA20230.10115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)