FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC43700.2185No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA41200.20600000000000002No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC37630.18814999999999998No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG35840.1792No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT32870.16435TruSeq Adapter, Index 1 (97% over 36bp)
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA27640.1382No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA23840.11919999999999999No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC23530.11765No Hit
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG22580.1129No Hit
CCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTCC20210.10104999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)