FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA152350.76175TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT89020.4451No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC85810.42905No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG61740.3087No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT61730.30865No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG52050.26025000000000004No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT48190.24095000000000003No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44830.22415No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44460.22230000000000003No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA39220.1961No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT37920.1896TruSeq Adapter, Index 1 (97% over 36bp)
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC37760.1888No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG37560.1878No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG37010.18505No Hit
GGGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTA35080.1754No Hit
GGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTAT33230.16615No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA32920.1646No Hit
GGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTAC32440.16219999999999998No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG31540.1577No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT30770.15385No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA28600.14300000000000002No Hit
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG27180.1359No Hit
GTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGGG26490.13244999999999998No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC26300.1315No Hit
CTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCT25210.12605No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT23610.11804999999999999No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG22970.11485No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG22320.1116No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA22230.11115000000000001No Hit
GCTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCT21160.10579999999999999No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC20690.10345No Hit
TGTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGG20020.1001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)