FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA99010.49505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT78430.39214999999999994TruSeq Adapter, Index 25 (97% over 37bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC65150.32575No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT56080.2804No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT40300.20149999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC38390.19195No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT34370.17185No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA30350.15175No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC26530.13265No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT22330.11165000000000001No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG21710.10855000000000001No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC21150.10575000000000001No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTGGTCAATCTCGTAT20940.1047Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)