FastQCFastQC Report
Fri 10 May 2024
EGAF00000927580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT131780.6589Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA39460.1973No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC28940.1447No Hit
AATTATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT25540.12769999999999998Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACACCTCCAAATCTCGTAT21170.10585Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAAACATCGATCTCGTAT20760.1038Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)