FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA213271.06635No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC66410.33205No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC64510.32255No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT63020.3151TruSeq Adapter, Index 7 (97% over 39bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT48390.24195000000000003No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA44260.22130000000000002No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT41970.20985No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG41150.20575No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG40640.2032No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT38270.19135Illumina Single End PCR Primer 1 (96% over 25bp)
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA37950.18975No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT35830.17915No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC34550.17275000000000001Illumina Single End Adapter 1 (96% over 25bp)
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT34530.17265No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG33130.16565No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC33010.16505No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC32810.16405No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC31830.15915No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC31140.1557No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG31130.15564999999999998No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC29060.1453No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT28050.14025No Hit
GTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCT27100.1355No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA26990.13495000000000001No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT25630.12815000000000001No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT24190.12095No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC24010.12004999999999999No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT21840.1092No Hit
CCCCTGGTTCGCTCCATACACCTATGTATTCAGTGTAAGGTAACCATCTT21290.10644999999999999No Hit
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT20820.1041Illumina Single End PCR Primer 1 (95% over 22bp)
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC20230.10115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)