FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000927624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA263831.31915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT109720.5486000000000001TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC88970.44485Illumina Single End Adapter 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC86190.43095No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC85100.4255No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT64840.3242No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG56610.28305No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA54280.2714No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT54140.2707No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG52850.26425No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC48820.2441No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC46590.23295No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT46330.23165No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC43090.21545000000000003No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA41160.20579999999999998No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT40530.20265No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG39510.19754999999999998No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC38070.19035No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT37890.18944999999999998No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT37260.18630000000000002No Hit
GTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCT35570.17784999999999998No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG35000.17500000000000002No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA34850.17425No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC31600.158No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT30220.15109999999999998No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC28280.1414No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC26340.1317Illumina Single End Adapter 1 (96% over 25bp)
CCCCTGGTTCGCTCCATACACCTATGTATTCAGTGTAAGGTAACCATCTT26240.1312No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC25180.12589999999999998No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTT25120.1256No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC25050.12525No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT24760.1238No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA24610.12305No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT24440.1222No Hit
CCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATG24390.12195No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGA24230.12115No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC23320.1166No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC22840.1142No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA22460.11230000000000001TruSeq Adapter, Index 7 (97% over 39bp)
GCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCC22180.1109No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA22100.11050000000000001No Hit
GTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA22000.11No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT21850.10925No Hit
CCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCT21810.10905000000000001No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT21800.109Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGC21580.10790000000000001No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT21180.10590000000000001No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC21030.10515No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT20960.1048Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT20730.10365No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGCACACGTCTGAAC20280.10139999999999999Illumina Single End Adapter 1 (96% over 25bp)
GCCCTTCAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAGGCTAGTT20150.10074999999999999No Hit
CCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCCT20090.10045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)