FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000927625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA138880.6944Illumina PCR Primer Index 9 (96% over 31bp)
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG117440.5871999999999999No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG53880.26940000000000003Illumina Single End PCR Primer 1 (100% over 30bp)
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG47090.23545No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA45600.22799999999999998No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG35470.17734999999999998No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG34750.17375No Hit
AGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTA32140.16069999999999998No Hit
GAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGG30410.15205No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT29440.1472No Hit
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACC28970.14484999999999998No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAGA28300.14150000000000001No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAG25530.12765No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG25040.1252No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT25020.1251No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA24360.12179999999999999No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC23140.1157Illumina Single End PCR Primer 1 (100% over 50bp)
CGGAGGAGTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTCGTAGAGT22570.11284999999999999No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC21880.1094No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG21820.1091No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA21170.10585No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT20980.10490000000000001Illumina Single End PCR Primer 1 (100% over 31bp)
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT20800.104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)