FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000927626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA257141.2857No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT108630.54315TruSeq Adapter, Index 7 (97% over 39bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC85580.4279No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC82490.41245No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC68990.34495Illumina Single End Adapter 1 (96% over 25bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT63550.31775000000000003No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG54670.27335No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT53090.26545No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG51760.25880000000000003No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA51750.25875No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC47140.23570000000000002No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT46180.2309No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC45530.22765No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC42730.21365No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT40760.20379999999999998No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA40320.2016No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG37120.1856No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT36760.1838No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC36200.181No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT36140.1807No Hit
GTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCT35000.17500000000000002No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG34400.172No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA34000.16999999999999998No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC32450.16225No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC30480.1524Illumina Single End Adapter 1 (96% over 25bp)
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT29110.14554999999999998No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC28280.1414No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT25180.12589999999999998No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA24980.12489999999999998No Hit
CCCCTGGTTCGCTCCATACACCTATGTATTCAGTGTAAGGTAACCATCTT24840.1242No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC24700.1235No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTT24370.12185No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGA24130.12065000000000001No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT23970.11984999999999998No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC23410.11705000000000002No Hit
CCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATG23310.11655000000000001No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC22590.11295000000000001No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC22590.11295000000000001No Hit
GTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA21890.10944999999999999No Hit
CCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCT21740.1087No Hit
GCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGC21700.1085No Hit
GCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCC21680.1084No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA21440.1072TruSeq Adapter, Index 7 (97% over 39bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA21390.10694999999999999No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT21280.10640000000000001No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC20940.1047No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT20800.104No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT20700.1035Illumina Single End PCR Primer 1 (96% over 25bp)
CCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCCT20450.10225No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT20330.10165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)