FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000927630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA259811.29905No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT109730.54865TruSeq Adapter, Index 7 (97% over 39bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC85320.4266No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC82560.41279999999999994No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC82030.41015Illumina Single End Adapter 1 (96% over 25bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT62940.3147No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG55070.27535No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT54620.2731No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG52960.26480000000000004No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA51740.2587No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT46230.23115000000000002No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC45960.2298No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC45160.2258No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC42760.21380000000000002No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA41130.20565000000000003No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT41050.20525000000000002No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG39190.19594999999999999No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC36640.1832No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT36370.18185No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT35360.1768No Hit
GTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCT35030.17515No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA34550.17275000000000001No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG33110.16555No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC31330.15664999999999998No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT29550.14775000000000002No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC28430.14215No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC26820.1341Illumina Single End Adapter 1 (96% over 25bp)
CCCCTGGTTCGCTCCATACACCTATGTATTCAGTGTAAGGTAACCATCTT25960.1298No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGA24960.1248No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT24940.1247No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC24920.12459999999999999No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTT24880.12440000000000001No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA24810.12405000000000001No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC24690.12344999999999999No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT24070.12035No Hit
CCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATG23770.11885000000000001No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC23220.1161No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC22540.1127No Hit
GCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGC22130.11065000000000001No Hit
CCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCT22110.11055No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA22040.11019999999999999TruSeq Adapter, Index 7 (97% over 39bp)
GCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCC21990.10995No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA21920.1096No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT21440.1072Illumina Single End PCR Primer 1 (96% over 25bp)
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT21300.1065No Hit
GTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA21010.10505No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC20880.1044No Hit
CCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCCT20370.10185000000000001No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT20340.10169999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT20290.10145No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT20180.10089999999999999No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT20140.10070000000000001No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGCACACGTCTGAAC20070.10035000000000001Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)