FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA306951.53475No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC147080.7354No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC76920.3846No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG66970.33485No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG45860.2293No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC39110.19555000000000003No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC35160.1758No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA33250.16624999999999998No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG31280.1564No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT30070.15035No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG27270.13635No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT26830.13415No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT25210.12605No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT23600.11800000000000001No Hit
TCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG23220.1161No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT22950.11475No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC22790.11395000000000001No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC22200.11100000000000002No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC21270.10635No Hit
TGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATT20280.10139999999999999No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT20140.10070000000000001TruSeq Adapter, Index 2 (97% over 38bp)
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT20110.10055000000000001No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT20040.10020000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)