FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000927860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA420702.1035000000000004No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC119890.59945No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG71540.35769999999999996No Hit
GCCTGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC57260.2863No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC57070.28535No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA56910.28454999999999997No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC53160.26580000000000004No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC49660.2483No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG49030.24515No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT44510.22255No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA41760.2088No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA38890.19445No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG38390.19195No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA35430.17715No Hit
TCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG35300.17650000000000002No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT32760.1638No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT31640.1582No Hit
GCCGTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA31130.15564999999999998No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT30380.1519No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG30160.1508No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG29530.14765No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC29240.1462No Hit
GGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT27810.13905No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG26170.13085No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA25510.12755No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT25370.12685000000000002No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC25290.12645No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC25050.12525No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT25030.12515No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG23730.11865No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT23670.11835000000000001No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT22930.11465No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT22910.11455No Hit
TGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATT22820.11410000000000001No Hit
CACTCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG22760.11379999999999998No Hit
ACTCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG22580.1129No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT22270.11134999999999999No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA22240.1112No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG22080.1104No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC21590.10794999999999999No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA21240.1062No Hit
CCGTTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC21140.1057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)