FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000927864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000927864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA224321.1216No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC143960.7198No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC68010.34004999999999996No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT51340.2567No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG45980.2299No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC43250.21625No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG42020.2101No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC38430.19215000000000002No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA37340.1867No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA36740.1837No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT36640.1832No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT36240.1812No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT35890.17945No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA35290.17645No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG32710.16355No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG32080.1604No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG29100.1455No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT28560.1428No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC28430.14215No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT27410.13705No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA26850.13424999999999998No Hit
GGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGAT26340.1317No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG26180.13090000000000002No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA25680.1284No Hit
GGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCT25360.1268No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC24810.12405000000000001No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC24680.12340000000000001No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT23930.11965No Hit
GTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCA23630.11815No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC23150.11574999999999999No Hit
GCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGT22040.11019999999999999No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT21750.10874999999999999No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA21700.1085No Hit
GGGAGAGATTTGGTATATGATTGAGATGGGGGCTAGTTTTTGTCATGTGA20420.1021No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT20400.10200000000000001No Hit
GCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTT20160.1008No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA20010.10005000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)