FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000928244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320004
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG19540.6106173672829089No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA17390.543430707116161No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA15010.46905663679204007No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA11190.349683128960888No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT10540.3293708828639642No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG7510.23468456644291946No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC7110.22218472269096637No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT6640.20749740628242147No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC5800.18124773440331995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT5750.17968525393432583TruSeq Adapter, Index 5 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA5470.17093536330795864No Hit
CTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGGTTG5050.1578105273684079No Hit
GGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGA5010.1565605429932126No Hit
CCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACAC4960.15499806252421844No Hit
CTCCAGCATTGTAGTTTGTAGCTCGTGTGTTGTAACCACTCTCCCATTTG4890.15281058986762666No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC3820.11937350783115211No Hit
GGCGTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTG3600.1124985937675779No Hit
CCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAG3440.10749865626679667No Hit
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG3240.10124873439082012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)