FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA30240.1512Illumina PCR Primer Index 9 (96% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC26740.1337Illumina Single End PCR Primer 1 (100% over 50bp)
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG20910.10455No Hit
GCGAGAATGAAGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAAAA20640.10319999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)