FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT79480.39740000000000003TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA58850.29424999999999996No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39310.19654999999999997No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT30220.15109999999999998Illumina Single End PCR Primer 1 (96% over 25bp)
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA26150.13075No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA24930.12465TruSeq Adapter, Index 7 (97% over 39bp)
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG24470.12235No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA23580.11789999999999999No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT20260.1013No Hit

[OK]Adapter Content

Adapter graph