FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT69620.3481TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA52280.2614No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT36430.18215Illumina Single End PCR Primer 1 (96% over 25bp)
AATTATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT32440.16219999999999998Illumina Single End PCR Primer 1 (95% over 22bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC25380.12689999999999999No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA23620.1181No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA23560.1178TruSeq Adapter, Index 7 (97% over 39bp)
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG22690.11345000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)