FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA122330.61165No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC117490.58745No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT84970.42484999999999995TruSeq Adapter, Index 9 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC60560.3028No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC31450.15725No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC31320.1566No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT29860.1493No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT28480.1424Illumina Single End PCR Primer 1 (96% over 25bp)
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG28380.1419No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA24520.12260000000000001TruSeq Adapter, Index 9 (97% over 38bp)
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG22920.11460000000000001No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT22800.11399999999999999No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT21340.1067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)