FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT62360.3118TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA50960.25479999999999997No Hit
AATTATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT26270.13135Illumina Single End PCR Primer 1 (95% over 22bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC25330.12665No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT24560.12279999999999999Illumina Single End PCR Primer 1 (96% over 25bp)
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA22510.11255No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG21170.10585No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA21140.1057TruSeq Adapter, Index 7 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)