FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT82310.41155TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA56250.28125No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT42640.21320000000000003Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37780.1889No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA26920.1346TruSeq Adapter, Index 7 (97% over 39bp)
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA25030.12515No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG24450.12225000000000001No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA22090.11045No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC21560.10779999999999999Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)