FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT71830.35915TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA60140.3007No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC42530.21265No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA26640.1332No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG24550.12275000000000001No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA24370.12185No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA22450.11225TruSeq Adapter, Index 7 (97% over 39bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT20730.10365No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC20650.10325No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA20650.10325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)