FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG52520.2626No Hit
GGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTAG52210.26105No Hit
GCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAG48150.24075No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA42170.21085No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37820.18910000000000002No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGC29020.1451No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA28020.1401No Hit
CCCCGCTGGAGCCCTGCTGGTTGCTGAGGTAGACGCTGAAGACGGGGCTG26790.13394999999999999No Hit
CTCACGGATAGACTTAAATTTCTTCAGGGGCACTTTGACCACTGCTGCCT23280.1164No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT22430.11215000000000001No Hit
GCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCTGA21910.10955No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA21510.10755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)