FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG53890.26944999999999997No Hit
GGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTAG53650.26825No Hit
GCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAG52140.2607No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA43500.21749999999999997No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC40450.20224999999999999No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGC30330.15165No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA28870.14434999999999998No Hit
CCCCGCTGGAGCCCTGCTGGTTGCTGAGGTAGACGCTGAAGACGGGGCTG28480.1424No Hit
CTCACGGATAGACTTAAATTTCTTCAGGGGCACTTTGACCACTGCTGCCT24920.12459999999999999No Hit
TGGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTA24140.1207No Hit
GCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCTGA23570.11785000000000001No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT23540.11770000000000001No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA22690.11345000000000001No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA20380.10189999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)