FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000928692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196822
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA9610.48825842639542333No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT6950.35311093272093563TruSeq Adapter, Index 9 (97% over 38bp)
GCCTGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC6210.31551350966863456No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC4080.20729390007214638No Hit
CCGTTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC3260.1656318907439209No Hit
AATTATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT2670.13565556695897815Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG2540.12905061426060097No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT2280.11584070886384652No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC2210.11228419587241265No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA2140.10872768288097875TruSeq Adapter, Index 9 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC2130.1082196095964882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)