FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA49020.2451No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44830.22415No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT34340.1717TruSeq Adapter, Index 1 (97% over 36bp)
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG25920.1296No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG24690.12344999999999999No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22610.11305No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA22530.11264999999999999No Hit
CTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATA21550.10774999999999998No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA20850.10425000000000001No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC20130.10065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)