FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000928990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG90830.45415000000000005No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA45510.22755000000000003No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT32880.16440000000000002No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG31000.155No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC28960.1448No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA27900.13949999999999999No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT26390.13194999999999998No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT21740.1087TruSeq Adapter, Index 7 (97% over 36bp)
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA21290.10644999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)