FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000928996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000928996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG87950.43975000000000003No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA55100.2755No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA48800.244No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA39370.19685000000000002No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT39070.19535No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA36310.18155000000000002No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG32470.16235No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC30040.1502No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT27830.13915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT23310.11655000000000001TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)