FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000929182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTA33310.16655TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT32890.16444999999999999TruSeq Adapter, Index 2 (97% over 38bp)
CCTGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT25950.12975No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA22710.11355No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT21720.10859999999999999No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC20110.10055000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)