FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000929265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT116610.58305TruSeq Adapter, Index 1 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA67040.3352No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC60680.3034No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT42270.21134999999999998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTA41670.20834999999999998TruSeq Adapter, Index 1 (97% over 38bp)
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA27080.1354No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC23660.1183No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22960.1148No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC21190.10594999999999999No Hit

[OK]Adapter Content

Adapter graph