FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000929317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095782
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA53660.4896959431711782No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC20990.19155269935078328No Hit
GCCTGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC17060.15568790142564853No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT17030.15541412434225058TruSeq Adapter, Index 5 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC16320.1489347333684985No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT15770.14391548683953562No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT13820.12611997641866723No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG12170.11106223683177859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)