FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000929407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA51890.25945No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT38550.19275TruSeq Adapter, Index 5 (97% over 37bp)
AATTATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT25230.12615Illumina Single End PCR Primer 1 (95% over 22bp)
GCCTGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC23140.1157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)