FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000929417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA80040.40020000000000006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT75290.37645TruSeq Adapter, Index 5 (97% over 37bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC56110.28054999999999997No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT28830.14415No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT24770.12385No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA23240.11620000000000001No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22190.11095000000000001No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC20680.10339999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)