FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000929419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243961
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA36150.29060396587995924No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT24920.20032782378225683TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT23280.18714413072435548Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC19340.1554711120364706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)