FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000929922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45740.2287No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45440.2272No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC38810.19405No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG36070.18035No Hit
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG30400.152No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT29070.14534999999999998TruSeq Adapter, Index 1 (97% over 36bp)
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA27340.1367No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC24990.12494999999999999No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA24670.12335No Hit
CTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATA20780.10389999999999999No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA20620.1031No Hit
GTAGGCTTCTTTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAA20520.1026No Hit
CCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGAT20030.10015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)