FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000929926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44780.22390000000000002No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA43410.21705000000000002No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC37760.1888No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG37040.1852No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT33440.16720000000000002TruSeq Adapter, Index 1 (97% over 36bp)
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG30210.15105No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA28150.14075No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC25460.1273No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA24900.1245No Hit
CTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATA20830.10415No Hit
GTAGGCTTCTTTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAA20700.1035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)