FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000929982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000929982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388882
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT25770.6626688815630448TruSeq Adapter, Index 15 (97% over 37bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTA5100.1311451802860508TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)