FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000930064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT72470.36235TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA72120.3606TruSeq Adapter, Index 5 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT66190.33094999999999997Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA57850.28925No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC41040.2052No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT31370.15685No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA21940.1097No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT21050.10525000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)