FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000930401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA264791.32395No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC157330.7866500000000001No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC79460.3973No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT76170.38084999999999997TruSeq Adapter, Index 1 (97% over 38bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA68970.34485No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC59710.29855No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT55430.27715No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG52420.2621No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT49960.24979999999999997Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC46170.23084999999999997No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA45150.22575No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT41340.2067No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT39290.19645No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG38540.19269999999999998No Hit
GTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGA33870.16935No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG33770.16885No Hit
GCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGA30810.15405No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT30490.15245No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT29480.1474No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT29170.14585No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA28920.1446No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG28250.14125No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA28150.14075No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC27960.13979999999999998No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCC27950.13975No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG27530.13765No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC26680.1334No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT26460.1323No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT26280.13140000000000002No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC26260.1313No Hit
GCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAA25230.12615No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC24900.1245No Hit
GTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAG24660.12329999999999999No Hit
GGGGGTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTT24620.12310000000000001No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG24580.12290000000000001No Hit
GTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAA23780.11889999999999999No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC23750.11875No Hit
GGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTAAAGAG23410.11705000000000002No Hit
CAAGCAGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTGCTT23320.1166TruSeq Adapter, Index 25 (96% over 28bp)
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT22570.11284999999999999No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT22550.11275No Hit
GCCGATGTTTCAGGTTTCTGAGTAGAGAAATGATCCGTAATATAGGCCTC22180.1109No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA21440.1072No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG20790.10395No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG20130.10065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)