FastQCFastQC Report
Thu 9 May 2024
EGAF00000930469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTAT31670.15835000000000002TruSeq Adapter, Index 8 (97% over 36bp)
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA28490.14245No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA20660.1033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)