FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000930827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT96350.48174999999999996TruSeq Adapter, Index 22 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA38500.1925No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC32950.16475No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC25270.12635000000000002No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA22960.1148TruSeq Adapter, Index 22 (97% over 38bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT20980.10490000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)