FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000930927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT234171.17085TruSeq Adapter, Index 8 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT71070.35535Illumina Single End PCR Primer 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTA50900.2545TruSeq Adapter, Index 8 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24590.12294999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)