FastQCFastQC Report
Wed 8 May 2024
EGAF00000930931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000930931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT143340.7167TruSeq Adapter, Index 8 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT87240.43620000000000003Illumina Single End PCR Primer 1 (96% over 25bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT50350.25175000000000003TruSeq Adapter, Index 5 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTA31700.1585TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)