FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT65310.32655TruSeq Adapter, Index 25 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA63080.3154No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC52130.26065No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC27090.13545000000000001No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT24230.12115Illumina Single End PCR Primer 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTA21860.1093TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)