FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA118010.5900500000000001TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT93720.4686No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC91470.45735000000000003No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG67290.33644999999999997No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT63720.3186No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG54230.27115No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT50200.251No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC46890.23445No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA46650.23324999999999999No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC39760.19880000000000003No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA39030.19515No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG38730.19365000000000002No Hit
GGGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTA38270.19135No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG37830.18914999999999998No Hit
GGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTAT36460.1823No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA35290.17645No Hit
GGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTAC33590.16795No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG33360.1668No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT33000.165No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA31350.15675No Hit
GTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGGG29920.14959999999999998No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT28370.14185TruSeq Adapter, Index 1 (97% over 36bp)
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG27790.13895No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC26830.13415No Hit
CTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCT26580.13290000000000002No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG24430.12215No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA22980.1149No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG22310.11155No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT22250.11125No Hit
GCCCCATCAATCCACTGCCACTGCTGCCTCTTCTGTGGGTCGTGCAGGCC21140.1057No Hit
TGTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGG21110.10555No Hit
GCTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCT21110.10555No Hit
GGGGTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAA21100.1055No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC21070.10535No Hit
GGGTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAG20050.10024999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)