FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA124460.6223000000000001TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT90160.4508No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC86600.43299999999999994No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT56630.28314999999999996No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG56080.2804No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG50890.25445No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT49230.24615No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA46700.23349999999999999No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45400.22699999999999998No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC37760.1888No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA36660.1833No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG36230.18115No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA33500.1675No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG32910.16455No Hit
GGGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTA32160.1608No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG31670.15835000000000002No Hit
GGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTAT31610.15805No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT29940.1497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT29640.1482TruSeq Adapter, Index 1 (97% over 36bp)
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA29450.14725000000000002No Hit
GGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTAC28320.1416No Hit
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG27090.13545000000000001No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC25910.12955No Hit
CTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCT24570.12285No Hit
GTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGGG24240.1212No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG24020.1201No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT22780.1139No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA21710.10855000000000001No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG21630.10815No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC21150.10575000000000001No Hit
GCTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCT20830.10415No Hit
TGTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGG20100.1005No Hit
GTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTATGT20060.1003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)