FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA157110.78555TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT87160.43579999999999997No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC87120.4356No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG50610.25305No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT47600.23800000000000002No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45860.2293No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44110.22055No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG41060.2053No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT40040.2002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT38080.1904TruSeq Adapter, Index 1 (97% over 36bp)
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA36730.18365No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC35820.1791No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG35810.17905No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA32150.16075No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG30220.15109999999999998No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA29170.14585No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT29140.1457No Hit
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG26770.13385No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC26060.1303No Hit
GTTGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG24950.12474999999999999No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG24720.12359999999999999No Hit
GGGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTA24120.1206No Hit
GGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTAT23820.1191No Hit
GGTAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT23530.11765No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG22590.11295000000000001No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA22360.11180000000000001No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG22270.11134999999999999No Hit
GGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTAC22150.11075No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT21720.10859999999999999No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC21400.107No Hit
GCTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCT20120.1006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)