FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA33390.16695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT32300.16149999999999998TruSeq Adapter, Index 15 (97% over 36bp)
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA31630.15815No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT25430.12715No Hit
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG22940.1147No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA21970.10985No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC21950.10975No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA20480.10239999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)